Changes in the gut bacteria composition associated with metabolic syndrome*

Authors

  • Anna Kotrova St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
  • Alexandr Shishkin St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation ; St. Petersburg Research Institute of Phthisiopulmonology, Health Ministry of Russia, 2–4, Ligovskiy pr., St. Petersburg, 191036, Russian Federation
  • Ivan Pchelin St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
  • Natalia Hudiakova St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
  • Nikita Gladyshev St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
  • Elena Ermolenko Institute of Experimental Medicine, 12, ul. Akademika Pavlova, St Petersburg, 197376, Russian Federation

DOI:

https://doi.org/10.21638/spbu11.2022.102

Abstract

Obesity, arterial hypertension, disorders of carbohydrate and lipid metabolism, which form the metabolic syndrome, remain the leading risk factors for the development of cardiovascular complications and oncological diseases. Overnutrition and a sedentary lifestyle leading to the metabolic syndrome formation are also interconnected with another potential pathogenetic factor of obesity, the gut microbiota. The study of its composition, as well as its metabolites, can serve as a basis for predicting metabolic disorders and for the development of an integrated approach to the metabolic syndrome treatment using probiotic therapy. The clinical assessment of the gut microbiota composition in patients with MS was carried out to identify bacterial taxa potentially associated with the development of MS. Materials (feces) were collected from 113 patients: the first group (n = 59) consisted of overweight patients (body mass index > 25.0) with metabolic disorders in the form of lipid and/or carbohydrate disorders (the average age 44 years). The control group (n = 54) consisted of patients with normal body weight (18.5 < body mass index < 25.0), without metabolic disorders, without arterial hypertension (the average age — 38 years). Fecal samples from patients were studied using 16S rRNA gene sequencing on the Illumina platform (MiSeq sequencer). The CDHIT-OTU-Miseq program was used to search for taxonomic units. Deoxyribonucleic acid libraries were prepared using the Illumina Nextera sample preparation kit with deoxyribonucleic acid primers corresponding to the V3–V4 regions of the 16S rRNA gene. In the gut microbiota composition of patients with metabolic syndrome we can highlight the phylum Actinobacteria, its 2 genera Actynomyces spp. and Bifidobacterium spp., as well as the genus Prevotella spp. and the class Gammaproteobacteria. It is assumed that bacterial species belonging to these type, class, and genera can be considered as potential marker bacteria of the metabolic syndrome.

Keywords:

gut microbiota, metabolic syndrome, obesity, 16s rRNA, Actinobacteria, Bifidobacterium

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References


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Published

2022-05-26

How to Cite

Kotrova, A., Shishkin, A. ., Pchelin, I. ., Hudiakova, N. ., Gladyshev, N. ., & Ermolenko, E. . (2022). Changes in the gut bacteria composition associated with metabolic syndrome*. Vestnik of Saint Petersburg University. Medicine, 17(1), 14–26. https://doi.org/10.21638/spbu11.2022.102

Issue

Vol. 17 No. 1 (2022)

Section

Internal medicine

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Articles of "Vestnik of Saint Petersburg University. Medicine" are open access distributed under the terms of the License Agreement with Saint Petersburg State University, which permits to the authors unrestricted distribution and self-archiving free of charge.