Analysis of the level of B-cell populations in patients with pulmonary sarcoidosis*

Анна Малкова; Игорь  Кудрявцев; Наталия  Басанцева; Юлия  Зинченко; Анна Старшинова; Леонид Чурилов; Петр Яблонский

doi:10.21638/spbu11.2019.416

Авторы

Анна Малкова Saint Petersburg State University
Игорь Кудрявцев St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Наталия Басанцева St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation ;St. Petersburg Research Institute of Phthisiopulmonology, Health Ministry of Russia, 2–4, Ligovskiy pr., St. Petersburg, 191036, Russian Federation
Юлия Зинченко St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation ;St. Petersburg Research Institute of Phthisiopulmonology, Health Ministry of Russia, 2–4, Ligovskiy pr., St. Petersburg, 191036, Russian Federation
Анна Старшинова National Medical Research Center named after V.A.Almazov, 2, ul. Akkuratova, St. Petersburg, 197341, Russian Federation
Леонид Чурилов St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation ;St. Petersburg Research Institute of Phthisiopulmonology, Health Ministry of Russia, 2–4, Ligovskiy pr., St. Petersburg, 191036, Russian Federation
Петр Яблонский St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation ;St. Petersburg Research Institute of Phthisiopulmonology, Health Ministry of Russia, 2–4, Ligovskiy pr., St. Petersburg, 191036, Russian Federation

DOI:

https://doi.org/10.21638/spbu11.2019.416

Аннотация

Sarcoidosis is a granulomatous disease of unknown etiology. The role of B-cells in pathogenesis of sarcoidosis was shown in several studies. The importance of B-lymphocytes activation in sarcoidosis can be confirmed by the efficacy of anti-CD20 treatment. However, there is no data about alteration of certain B-cells subsets. In this study changes in subsets of B-lymphocytes in patients with sarcoidosis (n=37) and a control group (n=30) are analyzed. In patients with sarcoidosis misbalance of B-naïve and B-memory cells was found, the level of B-naïve cells proved to be more than 70.00% and B memory cells less than 30.00% (sensitivity 76%, specificity 70%). Among regulatory B cells there was detected an elevation of CD24+++CD38+++ B cells more than 6.5% (sensitivity 91%, specificity 88%) and CD5+CD27-B cells than 12.45% (sensitivity 76%,specificity 80%). These results show, that in sarcoidosis pathogenesis are involved B-naïve and B-memory cells, that is characteristic for granulomatous diseases, and also regulatory B cells, that produce anti-inflammatory cytokines.

Ключевые слова:

sarcoidosis, autoimmunity, subsets, B cells, humoral immune response

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