The effect of orexin-A on the morphofunctional characteristics of LPS-stimulated microglial cells

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DOI:

https://doi.org/10.21638/spbu11.2019.411

Abstract

Microglial cells have a protective function and are essentially immunocytes present in the central nervous system. Activation of microglia results in the production of cytokines and trophic factors that can have a damaging or protective effect on brain cells. Orexin-A has anti-inflammatory and neuroprotective properties. Subcutaneous administration of orexin-A enhances the survival of mice with lipopolysaccharide-induced endotoxin shock, reducing the level of pro-inflammatory cytokines and chemokines. The aim of this study was to determine the effect of orexin-A administered intraventricularly on the morphological characteristics of microglial cells activated by intraperitoneal injection of lipopolysaccharide (LPS). With the introduction of lLPS, there is a change in the number of microglial cells in the somatosensory cortex, as well as in their morphology. When these indicators were analyzed after administration of orexin-A to animals against the background of LPS stimulation, no changes were detected.

Keywords:

microglia, orexin-A, microglial cells processuses

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References


References

Rock R.B., Peterson P.K. Microglia as a pharmacological target in infectious and inflammatory diseases of the brain. Neuroimmun. Pharmacol., 2006, vol. 1, no. 2, pp. 117–126.

Wei R., Jonakait G.M. Neurotrophins and the anti-inflammatory agents interleukin-4 (IL-4), IL-10, and IL-11 and transforming growth factor-B1 (TGF-B1) down-regulate T cell costimulatory molecules B7 and CD40 on cultured rat microglia. Neuroimmunol., 1999, no. 95, pp. 8–18.

Becquet L., Abad C., Leclercq M., Miel C., Jean L., Riou G., Couvineau A., Boyer O., Yossan-Var Tan. Systemic administration of orexin-A ameliorates established experimental autoimmune encephalomyelitis by diminishing neuroinflammation. Neuroinflammation, 2019, no. 16, p. 64.

Gencer M., Akbayır E., Şen M., Arsoy E., Yılmaz V., Bulut N., Tüzün E., Türkoğlu R. Serum orexin-A levels are associated with disease progression and motor impairment in multiple sclerosis. Neurol. Sci., 2019, vol. 40, no. 5, pp. 1067–1070.

Xiong X., White R.E., Xu L., Yang L., Sun X., Zou B., Pascual C., Sakurai T., Giffard R.G., Xie X. S.Mitigation of murine focal cerebral ischemia by the hypocretin/orexin system

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Published

2020-06-15

How to Cite

Runde , A. (2020). The effect of orexin-A on the morphofunctional characteristics of LPS-stimulated microglial cells. Vestnik of Saint Petersburg University. Medicine, 14(4), 302– 306. https://doi.org/10.21638/spbu11.2019.411

Issue

Vol. 14 No. 4 (2019)

Section

Neurology. Neurosurgery. Psychiatry

License

Articles of "Vestnik of Saint Petersburg University. Medicine" are open access distributed under the terms of the License Agreement with Saint Petersburg State University, which permits to the authors unrestricted distribution and self-archiving free of charge.