Features of lymphocyte differentiation in ANCA-associated and virus-associated glomerulonephritis in children

Olga Gurina; Elena Dementyeva; Arina Stepanova; Aleksandr Blinov; Olga Varlamova

doi:10.21638/spbu11.2021.401

Authors

Olga Gurina St Petersburg State Pediatric Medical University, 2, Litovskaya ul., St Petersburg, 194100, Russian Federation
Elena Dementyeva St. Petersburg State Pediatric Medical University, 2, Litovskaya ul., St. Petersburg, 194100, Russian Federation
Arina Stepanova St. Petersburg State Pediatric Medical University, 2, Litovskaya ul., St. Petersburg, 194100, Russian Federation
Aleksandr Blinov St. Petersburg State Pediatric Medical University, 2, Litovskaya ul., St. Petersburg, 194100, Russian Federation
Olga Varlamova St. Petersburg State Pediatric Medical University, 2, Litovskaya ul., St. Petersburg, 194100, Russian Federation

DOI:

https://doi.org/10.21638/spbu11.2021.401

Abstract

Glomerulonephritis is a heterogeneous group of immuno-inflammatory diseases. The purpose of the study: to analyze the subpopulation composition of lymphocytes in children with various types of glomerulonephritis. 50 children aged 1 to 16 years were examined: 1 group — 17 children aged 1 to 16 years with ANCA-associated glomerulonephritis; 2 group — 33 patients aged 2 to 16 years with virus-associated glomerulonephritis. Diagnostics of the subpopulation composition of lymphocytes was performed by flow cytometry using non-flushing technology using a three-color combination of monoclonal antibodies. It was found that the development of ANCA-associated glomerulonephritis is accompanied by signs of immunodeficiency against the background of a relative increase in the content of T-lymphocyte subpopulations. Differentiation of lymphocytes in children with virus-associated glomerulonephritis is ambiguous: the process goes either along the path of activation of antiviral protection, or along the path of development of insufficient antiviral activity of immunocytes. In children with virus-associated glomerulonephritis, a decrease in the level of B-lymphocytes was found, correlating with hypoimmunoglobulinemia A, which is an additional factor in reducing resistance on the mucous membranes. The study of lymphocyte differentiation in the development of glomerulonephritis is important for identifying the main immunological mechanisms involved in the pathogenesis of these diseases.

Keywords:

flow cytometry, lymphocyte subpopulations, lymphocyte phenotype, glomerulonephritis, children

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References

References

Dementyeva E. A. Subpopulation composition of lymphocytes in various types of glomerulonephritis in children. Medical Academic Journal, 2016, vol. 16, no. 4, pp. 103–104. (In Russian)

Dzeranova N., Isakov V., Kovalev Yu., Zotov D. Glomerulonephritis and psoriasis: psoriatic nephropathy or a random combination? (Brief review of the literature and clinical observation). University Therapeutic Journal,2019, vol. 1, no. 1, pp. 47–58. (In Russian)

Papayan A. V., Savenkova N. D. (eds). Clinical nephrology of childhood: A guide for doctors. St Petersburg: Levsha Publ., 2008. 600 p. (In Russian)

Savenkova N. D. ANCA-associated vasculitis in children. Nephrology (Saint-Petersburg), 2014, vol. 18, no. 3, pp. 16–23. https://doi.org/10.24884/1561-6274-2018-22-3-11-17 (In Russian)

Vanoni F., Bettinelli A., Keller F., Bianchetti M. G., Simonetti G. D. Vasculitides associated with IgG antineutrophil cytoplasmatic autoantibodies in childhood. Pediatr. Nephrol., 2010, vol. 25, no. 2, pp. 205–212. https://doi.org/10.1007/s00467-009-1253-3

Shenfeld I., Meroni P. L., Churilov L. P. (eds). Guide to autoimmune diseases for general practitioners. St Petersburg: Medkniga “ELBI” Publ., 2017. 416 p. (In Russian)

Necheporenko N. V., Savenkova N. D., Kalinina N. M. Clinical and immunologic characteristics and therapy of nephrotic syndrome associated with herpes infection types 1/2, 4, 5, in children with atopy.Nephrology (Saint-Petersburg), 2016, vol. 20, no. 3, pp. 69–77. (In Russian)

Necheporenko N. V., Savenkova N. D., Kalinina N. M. The role of herpesvirus infection in glomerulonephritis and nephrotic syndrome in children. Russian Bulletin of Perinatology and Pediatrics, 2017, vol. 62, no. 1, pp. 19–25. https://doi.org/10.21508/1027-4065-2017-62-1-19-25 (In Russian)

Necheporenko N. V., Kalinina N. M., Savenkova N. D. Glomerulonephritis associated with viral infection in children and adolescents. Nephrology (Saint-Petersburg), 2010, vol. 14, no. 2, pp. 35–45. (In Russian)

Gurina O. P., Dementieva E. A., Blinov A. E., et. al. Immunophenotype of lymphocytes in virus-associated glomerulonephritis in children. Modern pediatrics. St Petersburg — White Nights — 2018. Materials of the forum, 2018, pp. 38–39. (In Russian)

Wenderfer S. E. Viral-associated glomerulopathies in children.Pediatr. Nephrol., 2015, vol. 30, no. 11, pp. 1929–1938. https://doi.org/10.1007/s00467-015-3057-y

Ornstein B. W., Hill E. B., Geurs T. L., French A. R. Natural killer cell functional defects in pediatric patients with severe and recurrent herpesvirus infections. J. Infect. Dis., 2013, vol. 207, no. 3, pp. 458–468. https://doi.org/10.1093/infdis/jis701

Marleau A. M., Sarvetnick N. T-cell homeostasis in tolerance and immunity. J. Leukoc. Biol., 2005, vol. 78, no. 3, pp. 575–584. https://doi.org/10.1189/jlb.0105050

Kozlov V. A. Suppressor cells are the basis of immunopathogenesis of autoimmune diseases. Medical immunology (Russia), 2016, vol. 18, no. 1, pp. 7–14. https://doi.org/10.15789/1563-0625-2016-1-7-14 (In Russian)