Features of lymphocyte differentiation in ANCA-associated and virus-associated glomerulonephritis in children

Ольга  Гурина; Елена Дементьева; Арина Степанова; Александр Блинов; Ольга Варламова

doi:10.21638/spbu11.2021.401

Авторы

Ольга Гурина St Petersburg State Pediatric Medical University, 2, Litovskaya ul., St Petersburg, 194100, Russian Federation
Елена Дементьева St. Petersburg State Pediatric Medical University, 2, Litovskaya ul., St. Petersburg, 194100, Russian Federation
Арина Степанова St. Petersburg State Pediatric Medical University, 2, Litovskaya ul., St. Petersburg, 194100, Russian Federation
Александр Блинов St. Petersburg State Pediatric Medical University, 2, Litovskaya ul., St. Petersburg, 194100, Russian Federation
Ольга Варламова St. Petersburg State Pediatric Medical University, 2, Litovskaya ul., St. Petersburg, 194100, Russian Federation

DOI:

https://doi.org/10.21638/spbu11.2021.401

Аннотация

Glomerulonephritis is a heterogeneous group of immuno-inflammatory diseases. The purpose of the study: to analyze the subpopulation composition of lymphocytes in children with various types of glomerulonephritis. 50 children aged 1 to 16 years were examined: 1 group — 17 children aged 1 to 16 years with ANCA-associated glomerulonephritis; 2 group — 33 patients aged 2 to 16 years with virus-associated glomerulonephritis. Diagnostics of the subpopulation composition of lymphocytes was performed by flow cytometry using non-flushing technology using a three-color combination of monoclonal antibodies. It was found that the development of ANCA-associated glomerulonephritis is accompanied by signs of immunodeficiency against the background of a relative increase in the content of T-lymphocyte subpopulations. Differentiation of lymphocytes in children with virus-associated glomerulonephritis is ambiguous: the process goes either along the path of activation of antiviral protection, or along the path of development of insufficient antiviral activity of immunocytes. In children with virus-associated glomerulonephritis, a decrease in the level of B-lymphocytes was found, correlating with hypoimmunoglobulinemia A, which is an additional factor in reducing resistance on the mucous membranes. The study of lymphocyte differentiation in the development of glomerulonephritis is important for identifying the main immunological mechanisms involved in the pathogenesis of these diseases.

Ключевые слова:

flow cytometry, lymphocyte subpopulations, lymphocyte phenotype, glomerulonephritis, children

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