Mechanism of antisurdant action of triazin-indole derivative at experimental acoustic trauma

Authors

  • Vladimir Pastushenkov Farm-TRISAN Ltd, 16, Serebristii bul., St. Petersburg, 197227, Russian Federation
  • Maxim Kuznecov S. M. Kirov Military Medical Academy, 6, ul. Academika Lebedeva, St. Petersburg, 194044, Russian Federation
  • Vladimir Dvorjanchikov S. M. Kirov Military Medical Academy, 6, ul. Academika Lebedeva, St. Petersburg, 194044, Russian Federation
  • Alexander Pastushenkov North-Western State Medical University named after I. I. Mechnikov, 41, Kirochnaya ul., St. Petersburg, 191015, Russian Federation
  • Madin Gabaeva Kabardino-Balkarian State University named after H. M. Berbekov, 173, ul. Chernyshevskogo, Nalchik, Kabardino-Balkarian Republic, 360004, Russian Federation

DOI:

https://doi.org/10.21638/spbu11.2021.102

Abstract

In our research performed using model of acoustic trauma at experimental animals (female of hybrids F1 of CBA and C57BL/6 lines) influence of triazin-indole at expression level of hypoxia induced factor (HIF) in Corti’s organ was studied at its therapy application. As a reference drug cytoflavin was used. Investigated drug in the form of 1 % solution was introduced into animals parenterally in dosage 5, 7, 10 mg/kg with 2 hr intervals after acoustic impact. Injection of cytoflavin as reference drag was performed in 1.7 ml/kg dosage. Level of HIF expression in the drug of Corti’s organ was estimated using immunohistochemical method. It was found out that triazin-indole derivative increases HIF expression in Corti’s cells and in neurons of spiral ganglion at acoustical traumatic impact depending on drug dosage increase. Maximal HIF expression in Corti’s cells and in ganglions were noted at therapeutic dose of the drug 10 mg/kg. In control group and in the group with application citoflavin in dose 1.7 ml/kg minimal HIF expression was observed. According to obtained results of performed investigation the authors concluded that antisurdant property of triazin-indole derivative is realized through the influence on HIF metabolism (probably, by blockage of prolyl hydroxylase) and enables to consider it as a target molecular during the drug application.

Keywords:

hypoxia, induced fator, HIF-1, acoustical trauma, antihypoxic drugs, triasin-indole, expression, laboratory animals, mean cytochemical coefficient

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References


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Published

2021-04-01

How to Cite

Pastushenkov , V., Kuznecov , M., Dvorjanchikov , V., Pastushenkov , A. ., & Gabaeva , M. (2021). Mechanism of antisurdant action of triazin-indole derivative at experimental acoustic trauma. Vestnik of Saint Petersburg University. Medicine, 16(1), 13–19. https://doi.org/10.21638/spbu11.2021.102

Issue

Vol. 16 No. 1 (2021)

Section

Pathological physiology

License

Articles of "Vestnik of Saint Petersburg University. Medicine" are open access distributed under the terms of the License Agreement with Saint Petersburg State University, which permits to the authors unrestricted distribution and self-archiving free of charge.