DESTRUCTIVE EFFECT OF ATMOSPHERIC PRESSURE PULSED CORONA DISCHARGE PLASMA IN AIR ON BIOFILMS OF OPPORTUNISTIC BACTERIA

Oksana V. Rybal’chenko; Olga M. Stepanova; Olga G. Orlova; Alexander M. Astafiev; Valentina V. Kapustina; Elena N. Pariyskaya; Lidia B. Zacharova; Olga N. Vishnevskaya; Anatoly A. Kudryavtsev

doi:10.21638/11701/spbu11.2016.207

Authors

Oksana V. Rybal’chenko St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Olga M. Stepanova St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Olga G. Orlova St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Alexander M. Astafiev St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Valentina V. Kapustina St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Elena N. Pariyskaya St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Lidia B. Zacharova St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Olga N. Vishnevskaya St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Anatoly A. Kudryavtsev St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation

DOI:

https://doi.org/10.21638/11701/spbu11.2016.207

Abstract

Импульсный коронный разряд (ИКР) средней частоты в воздухе способен оказывать бактерицидное и бактериостатическое действие на культуру Escherichia сoli М17 как на клеточном, так и на популяционном уровне. Действие ИКР препятствует формированию микробного сообщества и приводит к деструкции уже образованных биопленок. В работе представлены данные электронно-микроскопических исследований ультраструктуры клеток и биопленок после их обработки ИКР в течение 90 секунд. Изменения морфологических свойств клеток E. сoli M17, вызванные обработкой ИКР, подобны изменениям после сублетального и летального теплового воздействия. Библиогр. 32 назв. Ил. 6.

Keywords:

pulsed corona discharge, atmospheric pressure discharge source, nonthermal air plasma, opportunistic bacteria, Escherichia coli, bacterial biofilm, cell destructive mechanisms

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