Bioavailability Study of Recombinant Plasmid DNA Nerve Growth Factor after Intramuscular Injection by Electroporation
DOI:
https://doi.org/10.21638/spbu11.2019.201Abstract
Comparative analysis of plasmid DNA delivery into cells efficiency (with effective agent concentration 250 μg/kg), ensuring accumulation of nerve growth factor (NGF) after intramuscular injection and intramuscular administration using electroporation method on rats was performed. Application of electroporation method ensured over 4 times increase of DNA plasmid concentration in muscle tissue within the first hour after its injection and increased the duration of its determination. Higher bioavailability parameters were confirmed by 3 times increase in gene expression level, encoding NGF accumulation and 3 times increase in concentration of the target NGF protein in blood plasma. The results obtained allow us to recommend this method for introduction of NGF plDNA in its biological activity and effectiveness in models of nerve damage investigation.
Keywords:
plasmid DNA, pharmacokinetics, gene expression, bioavailability, electroporation
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Articles of "Vestnik of Saint Petersburg University. Medicine" are open access distributed under the terms of the License Agreement with Saint Petersburg State University, which permits to the authors unrestricted distribution and self-archiving free of charge.