Single-nucleotide Polymorphisms of AGT, SOD2, CAT in Risk Estimates for Arterial Hypertension among Young People

Irina V. Astratenkova; Alexandra V. Kovaleva; Natalya E. Golovanova; Olga G. Orlova; Oksana V. Rybalchenko

doi:10.21638/spbu11.2019.206

Authors

Irina V. Astratenkova St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Alexandra V. Kovaleva St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Natalya E. Golovanova St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Olga G. Orlova St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Oksana V. Rybalchenko St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation

DOI:

https://doi.org/10.21638/spbu11.2019.206

Abstract

Arterial hypertension is one of the most common cardiovascular diseases and one of the main causes of mortality and disability of population. Opportunity to predict emergence of arterial hypertension and to take preventive measures in pre-nosological diagnostics of disease arouse research interest in this issue. Possibility to predict progression of arterial hypertension based on polymorphic variants of AGT gene (C521T, rs4762) identification, which encodes angiotensinogen protein in combination with polymorphic variants of CAT (–262C/T, rs1001179), encoding catalase, and polymorphic variants of SOD2 (C47T, rs4880), encoding mitochondrial superoxide dismutase, are discussed in this article. Decrease of antioxidant enzymes (catalase and superoxide dismutase) activity leads to accumulation of reactive oxygen species in cells. They may activate NF-kB transcription factor, which increases expression of individual genes involved in sodium homeostasis and in blood pressure control, including angiotensinogen. Increased angiotensinogen concentration in blood constitutes a risk factor in arterial hypertension development. In our opinion, combination of polymorphic variants of AGT, CAT and SOD genes expands the possibilities of personalized medicine.Performed investigation involved 65 medical students of St Petersburg State University. DNA was extracted from epithelial cells of the oral cavity. Genotyping of DNA samples was determined by real-time PCR. Obtained results of our investigation enabled to reveal AGT genotypes associated with hypertension in 12 students. The article discusses combinations of TT and CT genotypes of AGT with unfavorable alleles of antioxidant enzymes.Carriers of heterozygous SOD2 genotype and homozygous CC genotype CAT are in more unfavorable conditions compared to carriers of the CC genotype SOD2 and СТ genotype CAT, as SOD2 is a key antioxidant enzyme protecting from ROS.

Keywords:

arterial hypertension, genetic polymorphism, angiotensinogen, superoxide dismutase, catalase

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References

Bulgakova I. V., Bushueva O. Y., Polonikov A. V. Polymorphism a154c of cyp1a2 gene and risk of development of essential hypertension. Analyse of assosiation and relations with environmental risk factors. Fundamental research, 2013, iss. 9, pp. 994–998. (In Russian)

Timasheva Ya. R., Nasibullin T. R., Imaeva E. V., Mirsaeva G. Kh., Mustafina O. E. Beta‑adrenoreceptor gene polymorphisms and the risk of essential hypertension. Arterial’naya gipertenziya, 2015, vol. 21, iss. 3, pp. 259–266. https://doi.org/10.18705/1607-419X-2015-21-3-259-266 (In Russian)

Malyutina S. K., Maksimov V. N., Orlov P. S., Mazdorova E. V., Ryabikov A. N., Nikitin Yu. P.,Voevoda M. I. The association of blood pressure and hypertension with genetic markers identified in genome-wide association studies. Russian Journal of Cardiology, 2018, vol. 23, iss. 10, pp. 8–13. https://doi.org/10.15829/1560-4071-2018-10-8-13 (In Russian)

Obrezan A. G., Kulikov N. V. Neuro-humoral disbalance in chronic heart failure: classic and modern perspectives. Russian Journal of Cardiology, 2017, iss. 9, pp. 83–92. https://doi.org/10.15829/1560-4071-2017-9-83-92 (In Russian)

Sousa T., Oliveira S., Afonso J., Morato M., Patinha D., Fraga S., Carvalho F., Albino-Teixeira A. Role of H2O2 in hypertension, renin-angiotensin system activation and renal medullary disfunction caused by angiotensin II. British journal of Pharmocology, 2012, vol. 166, iss. 8, pp. 2386–2401. https://doi.org/10.1111/j.1476-5381.2012.01957.x

Wei Gu, Jielin Liu, Qiuli Niu, Hao Wang, Yuqing Lou, Kuo Liu, Lijuan Wang, Zuoguang Wang, Jingmei Zhang, Shaojun Wen. A-6G and A-20C Polymorphisms in the Angiotensinogen Promoter and Hypertension Risk in Chinese: A Meta-Analysis. PLoS One, 2011, vol. 6, iss. 12. https://doi.org/10.1371/journal.pone.0029489

Podolskаya А. А., Galyavich A. S., Maikova E. V., Kravtsova O. A., Alimova F. K. The role of antioxidant system genes in the formation of coronary heart disease clinical phenotypes. Kazan Medical Journal,2013, vol. 94, iss. 2, pp. 228–234. (In Russian)

Zhou X., Cui J., DeStefano A. L., Chazaro I., Farrer L. A., Manolis A. J., Gavras H., Baldwin C. T. Polymorphisms in the promoter region of catalase gene and essential hypertension. Disease Markers,2005, vol. 21, iss. 1, pp. 3–7. https://doi.org/10.1155/2005/487014