Влияние антимикробных веществ растительного происхождения на чувствительность бактериальных биопленок к антимикробным препаратам

Oksana  Rybalchenko; Olga Orlova; Maria  Netesa; Valentina  Kapustina

doi:10.21638/spbu11.2023.206

Authors

Oksana Rybalchenko St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Olga Orlova St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation
Maria Netesa Institute of Experimental Medicine, 2, ul. Akademika Pavlova, St. Petersburg, 197376, Russian Federation
Valentina Kapustina St. Petersburg State University, 7–9, Universitetskaya nab., St. Petersburg, 199034, Russian Federation

DOI:

https://doi.org/10.21638/spbu11.2023.206

Abstract

The widespread antibiotic resistance is associated with the formation of special forms of microbial communities — bacterial biofilms with increased resistance to the impact of various physicochemical factors, including antimicrobial drugs. In this regard, the investigation of antibacterial agents, including herbal preparations that could affect bacterial biofilms, seems to be of current interest. The aim of this work was to identify antibacterial substances of plant origin that can most effectively affect bacterial biofilms. The effect of antibacterial decoctions of lingonberry, sage, and chamomile plants on the sensitivity of bacteria to antimicrobial drugs was assessed. The susceptibility of bacteria to antimicrobial drugs, when combined with
herbal decoctions, was determined by the disk-diffusion method. The morphophysiological properties of biofilms were illustrated by light and scanning electron microscopy. The ability of lingonberry, sage, and chamomile decoctions to increase the sensitivity of bacteria to antimicrobial drugs of predominantly bactericidal pharmacological action was revealed. Decoctions of medicinal plants prevented the formation of complete bacterial biofilms. The obtained results indicate the antibiofilm activity of lingonberry, chamomile and sage decoctions, which contribute to a decrease in the level of antibiotic resistance of bacteria and
a decrease in the load during antimicrobial therap.

Keywords:

antibiotic resistance, antimicrobial drugs, bacterial biofilms, herbal decoctions

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References

Литература

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growth and biofilm formation in clinically relevant bacteria and yeasts. Biomed. Res. Int., 2014,vol. 2014, article ID 365272. https://doi.org/10.1155/2014/365272.

Song Y. J., Yu H. H., Kim Y. J., Lee N. K., Paik H. D. Anti-biofilm activity of grapefruit seed extract against staphylococcus aureus and Escherichia coli. J. Microbiol. Biotechnol., 2019, vol. 29 (8), pp. 1177–1183. https://doi.org/10.4014/JMB.1905.05022.

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O’Toole G. A. Microtiter dish biofilm formation assay. Journal of visualized experiments: JoVE, 2011,vol. 47, p. 2437. https://doi.org/10.3791/2437.